Yonsei Organelle
Molecular Sociology
[PHASE II]
세포 소기관 상호 작용을 규제하는 SM
소기관 근접성
물리화학적 평가 시스템
소기관 근접 물리화학적 평가 시스템(OPPAS)은 소기관 상호작용 조절자를 발견하는 데 유용한 도구입니다. OPPAS를 사용하면 소기관 간 6쌍의 근접성을 감지할 수 있습니다. 1단계 동안 우리는 소 기관 특이적 항체를 사용하여 이 시스템을 최적화하고 다양한 생체 활성 소기관 상호 작용 조절제를 성공적으로 발견했습니다.
1단계
소기관 근접성
물리화학적 평가 시스템
소기관 근접 물리화학적 평가 시스템(OPPAS)은 소기관 상호작용 조절자를 발견하는 데 유용한 도구입니다. OPPAS를 사용하면 소기관 간 6쌍의 근접성을 감지할 수 있습니다. 1단계 동안 우리는 소기관 특이적 항체를 사용하여 이 시스템을 최적화하고 다양한 생체 활성 소기관 상호 작용 조절제를 성공적으로 발견했습니다.
Mitochondria
- Endoplasmic Reticulum
Mitochondria and the endoplasmic reticulum form a platform called the mitochondria-associated membrane (MAM), that facilitates communication between the two organelles. The MAM regulates several physiological processes, including calcium transport, lipid synthesis, autophagy, and ER stress.
Mitochondria
- Lysosome
Mitochondria and lysosomes which are central organelles of cellular metabolism interact each other to coordinate bidirectional organelle dynamics. In particular, lysosomes directly regulate mitochondrial fission through contact sites. Mitochondria-Lysosome contacts also mediate transport of metabolites, lipids, calcium and iron.
Mitochondria
- Peroxisome
Membrane contact sites of mitochondria and peroxisomes contribute to β-oxidation of FAs and has the ability to convey information to each other through the release of biological messengers such as ROS, lipids, or other metabolites. In addition, these organelles undergo intimate crosstalk during cellular redox metabolism.
Endoplasmic Reticulum
- Plasma membrane
The endoplasmic reticulum directly contacts the plasma membrane, play a significant role in regulating Ca2+ signaling and maintaining homeostasis. When Ca2+ levels decrease, the STIM protein interacts with ORAI, initiating Store-Operated Calcium Entry (SOCE). Tethering between the ER and plasma membrane also regulates ion and lipid transport, signal transduction, ER morphology and remodeling.
Endoplasmic Reticulum
- Lysosome
The direct membrane contact site between the Endoplasmic Reticulum (ER) and Lysosome facilitates transport of ions, cholesterol, lipids. It also influences lysosomal positioning, fission, and the dephosphorylation of lysosome membrane receptors by ER-based phosphatases.
Endoplasmic Reticulum
- Golgi Apparatus
The endoplasmic reticulum and Golgi interact and play roles in ceramide transport, subcellular localization, activation of lipid reverse exchange, and regulation of protein trafficking. However, many unknowns remain regarding their regulation and role in controlling key functions such as sorting and trafficking and their relevance in physiological and pathological conditions.
